Pulsed photothermal radiometry (PPTR) can be used for non-invasive location of chromophores in strongly scattering biological samples. One interesting application is depth profiling of hypervascular lesions in human skin, such as port wine stain, where real-time determination of epidermal thickness and PWS depth should help optimize laser therapy on individual patient basis. I'll demonstrate how the strong spectral variation of the IR absorption coefficient of tissue in the commonly used detection band of 3-5 mm (InSb focal plane arrays) can be detrimental to the PPTR profiling, unless it is accounted for explicitly in the image reconstruction algorithm. Alternatively, its influence can be minimized by reducing the detection band. The latter approach has been implemented for profiling of PWS in human patients. When PWS starts in close proximity to the epidermis, the two layers may not be resolvable by PPTR. However, we can use two laser excitation wavelengths to separate the epidermal and vascular contribution to the radiometric signal based on their spectral differences. A recent refinement to this technique will be presented and discussed on an example of in vivo profiling of PWS. Some preliminary results of the use of such approach for 3-dimensional PPTR tomography of PWS will also be presented.